Proteins are complex biomolecules that require an intricate set of biochemical processes for their synthesis. Due to the innate complexity and size of proteins, chemical synthesis is often impractical for their production. Instead, the cellular machinery of living cells is harnessed as factories to assemble proteins based on supplied DNA templates. The resulting proteins are called recombinant proteins.More info :https://www.bosterbio.com/services/custom-antibody-production-services
Traditional strategies for protein expression involve the transfection of host cells with a DNA vector containing the gene template, followed by cell growth to enable transcription and translation to produce recombinant proteins. The proteins produced are then lysed and extracted for purification. The quality of a recombinant protein can be significantly influenced by the selection and culturing conditions used, as well as by factors such as folding and post-translational modifications (PTMs).
Protein Expression Service: Choosing the Right Provider for Your Project
The choice of which protein expression system to use is dictated by the characteristics of the target protein, including its function, structural properties, stability and aggregation properties. The table below outlines five different protein expression systems – mammalian, yeast, insect, bacteria and cell-free – and their advantages and challenges.
Sino Biological offers high-efficiency recombinant protein production using the baculovirus mediated insect cell expression system. This versatile system provides a high capacity for both intracellular and secreted protein expression, and it can be equipped with a range of functional tags to facilitate specific downstream applications. This platform also enables the expression of large, multi-protein constructs, as well as the incorporation of eukaryotic PTMs like complex glycosylation patterns.
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